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This study was carried out to investigate the possibility of utilization of a genetic marker for the improvement of a genetic ability in Holstein and Korean cattle, and analyzed the polymorphism for microsatellite of BoLA-DRB₃ intron₂ using PCR-RFLP after the isolation of genomic DNA from 323 cattle and also the analysis of association of allele types of microsatellites with economic traits. We obtained the PCR products of 170 to 220bp fragments and the distribution of genotypes in Holstein, Korean cattle, Charolais, Hereford, Angus, and Tiger-coated Korean cattle (14, 11, 7, 9, 8 and 6 allele types, respectively). The genotype of the most highest frequency was 185/185 genotype, except for Charolais. On the effect of microsatellite genotypes, association of AA (171/171), FF (189/189) and KK (219/219) genotypes as 7486.59, 7497.32 and 7464.50㎏ with 305-day milk yields in Holstein were significantly (P$lt;0.05) found to be higher than those of other genotypes. Also that of AA (171/171) and GH (193/195) genotype as 280.35 and 270.02㎏ with 18-month body weight showed significant (P$lt;0.05) in Korean cattle. Therefore, if the genetic markers defined relationship between the genetic polymorphism of BoLA-DRB₃ intron₂ and major economic traits, these could be used as a selection assisted tool for the genetic improvement in Holstein and Korean cattle.
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